{"id":2168,"date":"2026-03-02T09:43:57","date_gmt":"2026-03-02T07:43:57","guid":{"rendered":"https:\/\/krebs-feed.com.ua\/?page_id=2168"},"modified":"2026-03-03T15:25:16","modified_gmt":"2026-03-03T13:25:16","slug":"vyznachennya-viruliczydnoyi-diyi-preparatu-dez%c9%90-ultra-na-modeli-virusu-nyukaslskoyi-hvoroby","status":"publish","type":"page","link":"https:\/\/krebs-feed.com.ua\/en\/vyznachennya-viruliczydnoyi-diyi-preparatu-dez%c9%90-ultra-na-modeli-virusu-nyukaslskoyi-hvoroby\/","title":{"rendered":"DETERMINATION OF THE VIRULICIDAL EFFECT OF THE PREPARATION \u00abDES\u2c6f ULTRA\u00bb ON THE NEWCASTLE DISEASE VIRUS MODEL"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2168\" class=\"elementor elementor-2168\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4adec8f e-flex e-con-boxed wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-parent\" data-id=\"4adec8f\" 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elementor-widget-text-editor\" data-id=\"fbf1bc0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<a style=\"color: red;\" href=\"https:\/\/krebs-feed.com.ua\/en\/\">Home ,<\/a> <a style=\"color: red;\" href=\"https:\/\/krebs-feed.com.ua\/en\/business\/\">Business<\/a> \u2013 DETERMINATION OF THE VIRULICIDAL EFFECT OF THE PREPARATION \u00abDES\u2c6f ULTRA\u00bb ON THE NEWCASTLE DISEASE VIRUS MODEL\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-bbbe74a e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-parent\" data-id=\"bbbe74a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-3d4e942 e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no 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data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><span class=\"wpr-label\">Nov 09, 2024<\/span><\/time><div class=\"wpr-timeline-entry-inner\"><div class=\"wpr-main-line-icon wpr-icon\"><i aria-hidden=\"true\" class=\"far fa-flag\"><\/i><\/div><div class=\"wpr-story-info-vertical wpr-data-wrap\"  data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><div class=\"wpr-animation-wrap wpr-timeline-media\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1041\" height=\"348\" src=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1.png\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1.png 1041w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-300x100.png 300w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-1024x342.png 1024w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-768x257.png 768w\" sizes=\"(max-width: 1041px) 100vw, 1041px\" \/><\/div><div class=\"wpr-timeline-content-wrapper\"><div class=\"wpr-content-wrapper\"><div class=\"wpr-title-wrap\"><a href=\"https:\/\/krebs-feed.com.ua\/en\/vyznachennya-viruliczydnoyi-diyi-preparatu-dez%c9%90-ultra-na-modeli-virusu-nyukaslskoyi-hvoroby\/\" target=\"_blank\" rel=\"nofollow\"><span class=\"wpr-title\">DETERMINATION OF THE VIRULICIDAL EFFECT OF THE PREPARATION \u00abDES\u2c6f ULTRA\u00bb ON THE NEWCASTLE DISEASE VIRUS MODEL<\/span><\/a><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/article><article class=\"wpr-timeline-entry wpr-right-aligned elementor-repeater-item-f98ca99\" data-item-id=\"elementor-repeater-item-f98ca99\"><time class=\"wpr-extra-label\" data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><span class=\"wpr-label\">Apr 29, 2024<\/span><\/time><div class=\"wpr-timeline-entry-inner\"><div class=\"wpr-main-line-icon wpr-icon\"><i aria-hidden=\"true\" class=\"far fa-paper-plane\"><\/i><\/div><div class=\"wpr-story-info-vertical wpr-data-wrap\"  data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><div class=\"wpr-animation-wrap wpr-timeline-media\"><img decoding=\"async\" width=\"836\" height=\"445\" src=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/ptax_for-1.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/ptax_for-1.jpg 836w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/ptax_for-1-300x160.jpg 300w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/ptax_for-1-768x409.jpg 768w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/ptax_for-1-18x10.jpg 18w\" sizes=\"(max-width: 836px) 100vw, 836px\" \/><\/div><div class=\"wpr-timeline-content-wrapper\"><div class=\"wpr-content-wrapper\"><div class=\"wpr-title-wrap\"><a href=\"https:\/\/krebs-feed.com.ua\/en\/mizhnarodnyj-forum-poultry-farming\/\" target=\"_blank\" rel=\"nofollow\"><span class=\"wpr-title\">International Poultry Farming Forum<\/span><\/a><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/article><span class=\"wpr-year-wrap\"><span class=\"wpr-year-label wpr-year\">2024<\/span><\/span><article class=\"wpr-timeline-entry wpr-left-aligned elementor-repeater-item-1a24a71\" data-item-id=\"elementor-repeater-item-1a24a71\"><time class=\"wpr-extra-label\" data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><span class=\"wpr-label\">Apr 19, 2024<\/span><\/time><div class=\"wpr-timeline-entry-inner\"><div class=\"wpr-main-line-icon wpr-icon\"><i aria-hidden=\"true\" class=\"far fa-lightbulb\"><\/i><\/div><div class=\"wpr-story-info-vertical wpr-data-wrap\"  data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><div class=\"wpr-animation-wrap wpr-timeline-media\"><img decoding=\"async\" width=\"836\" height=\"402\" src=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/roz_pr-1.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/roz_pr-1.jpg 836w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/roz_pr-1-300x144.jpg 300w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/roz_pr-1-768x369.jpg 768w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/03\/roz_pr-1-18x9.jpg 18w\" sizes=\"(max-width: 836px) 100vw, 836px\" \/><\/div><div class=\"wpr-timeline-content-wrapper\"><div class=\"wpr-content-wrapper\"><div class=\"wpr-title-wrap\"><a href=\"https:\/\/krebs-feed.com.ua\/en\/rozigrash-pryziv\/\" target=\"_blank\" rel=\"nofollow\"><span class=\"wpr-title\">Prize draw<\/span><\/a><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/article><article class=\"wpr-timeline-entry wpr-right-aligned elementor-repeater-item-9408e17\" data-item-id=\"elementor-repeater-item-9408e17\"><time class=\"wpr-extra-label\" data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><span class=\"wpr-label\">Apr 12, 2024<\/span><\/time><div class=\"wpr-timeline-entry-inner\"><div class=\"wpr-main-line-icon wpr-icon\"><i aria-hidden=\"true\" class=\"fas fa-bolt\"><\/i><\/div><div class=\"wpr-story-info-vertical wpr-data-wrap\"  data-aos=\"fade\" data-aos-left=\"\" data-aos-right=\"\" data-animation-offset=\"150\" data-animation-duration=\"600\"><div class=\"wpr-animation-wrap wpr-timeline-media\"><img loading=\"lazy\" decoding=\"async\" width=\"836\" height=\"231\" src=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/farming.png\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/farming.png 836w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/farming-300x83.png 300w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/farming-768x212.png 768w\" sizes=\"(max-width: 836px) 100vw, 836px\" \/><\/div><div class=\"wpr-timeline-content-wrapper\"><div class=\"wpr-content-wrapper\"><div class=\"wpr-title-wrap\"><a href=\"https:\/\/krebs-feed.com.ua\/en\/zaproshuyemo-vas-na-mizhnarodnyj-praktychnyj-forum-po-ptahivnycztvu\/\" target=\"_blank\" rel=\"nofollow\"><span class=\"wpr-title\">We invite you to the international practical forum on poultry farming<\/span><\/a><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/article><\/div>    \n\t\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b3805ab e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-child\" data-id=\"b3805ab\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ebab7ce elementor-widget elementor-widget-heading\" data-id=\"ebab7ce\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">DETERMINATION OF THE VIRULICIDAL EFFECT OF THE PREPARATION \u00abDES\u2c6f ULTRA\u00bb ON THE NEWCASTLE DISEASE VIRUS MODEL<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-88eafe2 elementor-widget elementor-widget-image\" data-id=\"88eafe2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"342\" src=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-1024x342.png\" class=\"attachment-large size-large wp-image-1429\" alt=\"\" srcset=\"https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-1024x342.png 1024w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-300x100.png 300w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1-768x257.png 768w, https:\/\/krebs-feed.com.ua\/wp-content\/uploads\/2026\/02\/cert1.png 1041w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e1bee5f elementor-widget elementor-widget-text-editor\" data-id=\"e1bee5f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><strong>From:\u00a0 <\/strong>Nov 09, 2024<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9405665 elementor-widget elementor-widget-text-editor\" data-id=\"9405665\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><strong>Tkachenko Semen<\/strong><br \/>Candidate of Veterinary Sciences, Acting Head of the Sector for the Study of Bacterial Diseases of Poultry<br \/><a style=\"color: red;\" href=\"mailto:semen270181@gmail.com\">semen270181@gmail.com<\/a>,<\/p><p><strong>Rula Oleksandr<\/strong><br \/>Candidate of Veterinary Sciences, Acting Head of the Department of Poultry Diseases<br \/><a style=\"color: red;\" href=\"mailto:aleksrula75@gmail.com\">aleksrula75@gmail.com<\/a>,<br \/><strong>Maxim Tymoshenko<\/strong><br \/>director<br \/><strong>Music Denis<\/strong><br \/>Doctor of Science, Senior Researcher<br \/><a style=\"color: red;\" href=\"mailto:dmuzyka77@gmail.com\">dmuzyka77@gmail.com<\/a><br \/><strong>National Scientific Center &quot;Institute of Experimental and Clinical<\/strong><br \/><strong>of Veterinary Medicine&quot; NAAS, Kharkiv<\/strong><br \/><strong>Fire Group LLC, Kyiv<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c93e557 elementor-widget elementor-widget-heading\" data-id=\"c93e557\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Innovations in poultry farming<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4306425 elementor-widget elementor-widget-text-editor\" data-id=\"4306425\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>One of the main links in the system of preventing the occurrence and spread of Newcastle disease is monitoring and prevention. At the same time, an important stage in the system of veterinary and sanitary measures is the disinfection of veterinary control facilities. A number of disinfectants have been developed and proposed for practical use. Large-scale production and implementation of disinfectants in practice is not possible without prior laboratory assessment of their virucidal properties.<\/p><p>The aim of our work was to study the virucidal properties of the new aldehyde disinfectant &quot;Des\u2c6f Ultra&quot; on a test model of the Newcastle disease virus (NDV).<\/p><p>The NH virus has been stored in the museum of the laboratory for the study of poultry diseases since 1972, deposited in the National Center &quot;IEKVM&quot; under number 7-02, when cultivated on chicken embryos (CE) it causes the accumulation of hemagglutinins in titers of 1:1024 - 1:2028. Infectious activity of the virus<br \/>is 11.36 EID\/0.2 cm3.<\/p><p><strong>\u00a0 \u00a0The study was conducted using two methods.<\/strong>\u00a0\u2013 suspension and contact-suspension.<\/p><p>Suspension method. The principle of the method is the ability of a disinfectant in certain dilutions to neutralize the infectious properties of the virus in a certain volume.<\/p><p>A virus dilution (10-1, with the addition of antibiotics) was prepared in phosphate-saline buffer, then the disinfectant under study was added to the obtained virus dilution at a final concentration of 0.5; 1; 2 and 3 % and further sequential dilutions were made to 10-3. Before use on KE, the mixture of virus and drug was kept for 20 min. Infection and KE dissection were carried out according to generally accepted requirements. Ovoscopy was performed daily twice a day.<\/p><p>The selected extraembryonic fluid (EEF) was tested for the presence of hemagglutinating virus in the hemagglutination reaction (HAR) with 1 % suspension of rooster erythrocytes. The HAR was performed in V-shaped plates according to the generally accepted method.<\/p><p>A total of two consecutive passages were performed on developing KE. For the second passage, native KE EER from the first passage with antibiotic was used. Infection of KE was performed as described above.<\/p><p>Analysis of the results obtained shows that the drug &quot;DezV Ultra&quot; starting from the working concentration of 1% and above with an exposure of 30 min has virucidal properties. Incomplete disinfection and virus replication occurs when using drug concentrations from 0.0005% (maximum dilution of the disinfectant) to 0.5%. In control samples without disinfectant, hemagglutination in the EER of KE was present.<\/p><p>Contact-suspension method. The principle of the method is the ability of a disinfectant on the surfaces of test objects to neutralize the infectious properties of the virus with a biological load (a mixture of bovine serum and virus).<\/p><p>After receiving positive results from previous studies, the final determination of the virucidal action of the drug &quot;DezV Ultra&quot; against the NX virus was carried out using test objects, namely: plates made of unpainted wood, metal and tiles (size 100x100mm). A mixture of the virus and bovine serum diluted in PBS 1:2 was applied to the test objects. After 10-15 min after the applied mixture dried, the drug was sprayed in final concentrations of 0.5, 1, 2 and 3 %. After 30, 60, 90 and 120 min, a sterile applicator was used to wash it off, transfer it to a bottle with a solution of antibiotics on PBS and after contact (20 min) infection with KE was carried out as indicated above.<\/p><p>Thus, as a result of the conducted studies, it was established that the disinfectant &quot;Des\u2c6f Ultra&quot; in concentrations of 0.5; 0.1; 2.0 and 3.0 % with an exposure of 30 minutes already disinfects test objects that were contaminated with the NH virus. On control test objects without the addition of disinfectant, hemagglutination in the EER of KE was present throughout the entire study period (up to 120 min).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1c6496c elementor-widget elementor-widget-heading\" data-id=\"1c6496c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Link to the source of information<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5246855 elementor-widget elementor-widget-text-editor\" data-id=\"5246855\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<a style=\"color: red;\" href=\"http:\/\/avianua.com\/conference\/4-onlajn-conferenciya-po-ptahivnictvu-2024.pdf\" rel=\"nofollow noopener\" 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2024 \u041b\u0438\u0441 09, 2024\u0412\u0418\u0417\u041d\u0410\u0427\u0415\u041d\u041d\u042f \u0412\u0406\u0420\u0423\u041b\u0406\u0426\u0418\u0414\u041d\u041e\u0407 \u0414\u0406\u0407 \u041f\u0420\u0415\u041f\u0410\u0420\u0410\u0422\u0423 \u00ab\u0414\u0415\u0417\u2c6f \u0423\u041b\u042c\u0422\u0420\u0410\u00bb \u041d\u0410 \u041c\u041e\u0414\u0415\u041b\u0406 \u0412\u0406\u0420\u0423\u0421\u0423 \u041d\u042c\u042e\u041a\u0410\u0421\u041b\u0421\u042c\u041a\u041e\u0407 \u0425\u0412\u041e\u0420\u041e\u0411\u0418 \u041a\u0432\u0456 29, 2024\u041c\u0456\u0436\u043d\u0430\u0440\u043e\u0434\u043d\u0438\u0439 \u0444\u043e\u0440\u0443\u043c Poultry Farming 2024 \u041a\u0432\u0456 19, 2024\u0420\u043e\u0437\u0456\u0433\u0440\u0430\u0448 \u043f\u0440\u0438\u0437\u0456\u0432 \u041a\u0432\u0456 12, 2024\u0417\u0430\u043f\u0440\u043e\u0448\u0443\u0454\u043c\u043e \u0412\u0430\u0441 \u043d\u0430 \u043c\u0456\u0436\u043d\u0430\u0440\u043e\u0434\u043d\u0438\u0439 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\u0443\u043f\u0440\u043e\u0434\u043e\u0432\u0436 \u0432\u0441\u044c\u043e\u0433\u043e \u043f\u0435\u0440\u0456\u043e\u0434\u0443 \u0434\u043e\u0441\u043b\u0456\u0434\u0436\u0435\u043d\u043d\u044f (\u0434\u043e 120 \u0445\u0432). \u041f\u043e\u0441\u0438\u043b\u0430\u043d\u043d\u044f \u043d\u0430 \u0434\u0436\u0435\u0440\u0435\u043b\u043e \u0456\u043d\u0444\u043e\u0440\u043c\u0430\u0446\u0456\u0457 http:\/\/avianua.com\/conference\/4-onlajn-conferenciya-po-ptahivnictvu-2024.pdf \u041f\u043e\u043f\u0435\u0440\u0435\u0434\u043d\u0456\u0439 \u043f\u043e\u0441\u0442 \u041c\u0456\u0436\u043d\u0430\u0440\u043e\u0434\u043d\u0438\u0439 \u0444\u043e\u0440\u0443\u043c Poultry 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